effects of combination of g-csf and scf one week prior to liver injury in acute liver damage model induced by thioacetamide administration

background: there are many reports regarding to effects of granulocyte colony-stimulating factor (g-csf) and stem cell factor (scf)alone in liver repair .but conflicting data have been reported regarding the role of growth factors such as g-csf and scf in the liver regeneration system. also, there is not such data regarding to effects of co-administration both of g-csf and scf in the liver damage condition. materials and methods: an experimental model of rat liver damage induced by the thioacetamide. five different groups of animals receiving 0.9% nacl, taa alone, taa + g-csf, taa + scf and taa + (g-csf+scf ). the activity of glutamate pyruvate transaminase (gpt/alt)and glutamate oxaloacetate transaminase (got/ast) were measured after the thioacetamide (taa) injection and the administration of combination of g-csf + scf for 12 weeks. also histological tests were carried out at the end experiments. results: the pre-treatment of combination of g-csf and scf for 12 weeks reduced the degree of liver injury. the mean of got activity was 61.24 (u/l) in the g-csf +scf and versus 132.86 in the taa-alone group. these differences in the got activity were statistically significant (p<0.05). also, in the g-csf +scf and taa group the mean of gpt activity (4.35 versus 11.79, respectively) were lower than in the taa-alone group, this difference was statistically significant (p<0.05). liver sections from a rat treated only with taa, showing damage, but taa and g-csf + scf no significant damage is present. on the other hand histological results revealed a very mild degree of inflammation were observed in the livers of the combination of g-scf+scf and taa-treated rats compared to taa only treated group. conclusion: biochemical and microscopic analysis revealed that combination of g-csf and scf pre-treatment significantly enhances liver regeneration after taa –induced liver injury.